Isolation of Embryonic Fibroblasts from Primary Cultures of Mouse Embryos

Custom Model Generation Services
Preclinical Testing Services
Anti-Tumor Drug Efficacy EvaluationAutoimmune Disease Efficacy EvaluationMetabolic Disease Efficacy EvaluationNeurological Disease Efficacy EvaluationCardiovascular Diseases Efficacy EvaluationBioanalytical ServicesPhenotyping ServicesNon-GLP Toxicity Assessment ServicesCell Therapy & Gene Therapy Efficacy EvaluationDisease Rat Models
Germ-Free and Microbiome Services
Mouse-Derived Gut Microbiota LibraryCustomized Germ-free ServicesEvaluation of Anti-tumor Effect of Gut MicrobiotaMicrobiota Extraction and TransplantationGerm-free Rederivation and BreedingMicrobial Strain Culture and Colonization
Cell Lines and Customization Services
Cell Line Customization ServicesSyngeneic Cell LinesReporter Cell LinesCDX Cell Lines
Patient Derived Xenograft (PDX) Mouse Models and Efficacy Services
Custom Breeding Services
Customized Mice Breeding ServicesCryopreservation and ResuscitationAssisted ReproductionMouse Rederivation Service
Animal Quality Testing Services
SNP Testing ServicesMicrobiology Testing ServicesGenetic IntegrityAnimal HealthAnimal Welfare
NeoMab Fully-Human Antibody Discovery Service
Assay Capabilities
Molecular PlatformPK/PD PlatformPathological Diagnosis PlatformCell-based Service PlatformFlow Cytometry PlatformImaging and Analysis PlatformBehavioral Analysis Platform
Other Services
Animal Facility and HusbandryReproductive and Embryology Services Isolation of Embryonic Fibroblasts

图片1(1).png

Mouse embryonic fibroblasts

Strengths

Strict quality control and assurance: Bacterial, fungal, and mycoplasma tests are carried out to guarantee cells are clean and free of contamination.

Rich experience: GemPharmatech has years of experience preparing embryo fibroblasts.

Multiple products in stock: Stocks of mouse embryo fibroblasts engineered with neomycin, hygromycin or puromycin are available for in-house use and externa customers.


Outline of experimental procedure

Mouse embryos are collected at E13.5 gestational age. Trunk tissue are processed with enzymatic digestion in the presence of chelating agent (usually EDTA) to obtain single-cell embryo fibroblasts. These are cultured in MEF growth medium so that fibroblasts can survive, grow, and proliferate.

图片2.png

Isolation of fibroblasts from mouse embryos and preparation of feeder layer cells


图片3.png

Mouse embryos at 13.5-day gestational age


图片1(2).png图片1.png

Mouse embryonic fibroblasts